Optical trapping of a spherically symmetric sphere in the ray-optics regime: a model for optical tweezers upon cells.
نویسندگان
چکیده
Since their invention in 1986, optical tweezers have become a popular manipulation and force measurement tool in cellular and molecular biology. However, until recently there has not been a sophisticated model for optical tweezers on trapping cells in the ray-optics regime. We present a model for optical tweezers to calculate the optical force upon a spherically symmetric multilayer sphere representing a common biological cell. A numerical simulation of this model shows that not only is the magnitude of the optical force upon a Chinese hamster ovary cell significantly three times smaller than that upon a polystyrene bead of the same size, but the distribution of the optical force upon a cell is also much different from that upon a uniform particle, and there is a 30% difference in the optical trapping stiffness of these two cases. Furthermore, under a small variant condition for the refractive indices of any adjacent layers of the sphere, this model provides a simple approximation to calculate the optical force and the stiffness of an optical tweezers system.
منابع مشابه
Investigation of inclined dual-fiber optical tweezers for 3D manipulation and force sensing.
Optical tweezers provide a versatile tool in biological and physical researches. Optical tweezers based on optical fibers are more flexible and ready to be integrated when compared with those based on microscope objectives. In this paper, the three-dimensional (3D) trapping ability of an inclined dual-fiber optical tweezers is demonstrated. The trapping efficiency with respect to displacement i...
متن کاملmeasuring viscoelastic properties of Red Blood Cell using optical tweezers
Efforts have been made to study the behavior of complex materials in micrometer dimensions with various techniques. One of these methods is the use of optical tweezers for biophysical applications. Red blood cells, as the most abundant blood-forming cells, play an important role in the life of living organisms, and their unique mechanical properties are important. In this report, the study of s...
متن کاملQuantum Squeezed Light Propagation in an Optical Parity-Time (PT)-Symmetric Structure
We investigate the medium effect of a parity-time (PT)-symmetric bilayer on the quantum optical properties of an incident squeezed light at zero temperature (T=0 K). To do so, we use the canonical quantization approach and describe the amplification and dissipation properties of the constituent layers of the bilayer structure by Lorentz model to analyze the quadrature squeezing of the outgoing ...
متن کاملManipulation and Trapping of Particles in the Ray Optics Regime Using DPS-DNG Layered Structures: Toward Calibration and Precision of Optical Tweezers
Optical traps or tweezers use the forces exerted by structured beams of light to confine and manipulate non-intrusively and non-destructively microscopic objects including living cells and bacteria, with high accuracy. This technique provides unique means to control the dynamics of small particles and played a revolutionary role in areas of the physical and biological sciences. This manuscript ...
متن کاملMultiplying optical tweezers force using a micro-lever.
This study presents a photo-driven micro-lever fabricated to multiply optical forces using the two-photon polymerization 3D-microfabrication technique. The micro-lever is a second class lever comprising an optical trapping sphere, a beam, and a pivot. A micro-spring is placed between the short and long arms to characterize the induced force. This design enables precise manipulation of the micro...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied optics
دوره 45 16 شماره
صفحات -
تاریخ انتشار 2006